Singh D;Rai S K;Pandey R S;Srivastava S; Mishra R K;Chaudhary S;Sharma S;Sushil Kumar
008114 Singh D;Rai S K;Pandey R S;Srivastava S; Mishra R K;Chaudhary S;Sharma S;Sushil Kumar (National Institute for Plant Genome Research, Jawaharlal Nehru Univ, Post Box. No 10531,Campus, Aruna Asaf Ali Marg, New Delhi-110 067, Email: email@example.com) : Predominance of the serpentine route in monoterpenoid indole alkaloid pathway of Catharanthus roseus. Proc Indian Natn Sci Acad 2008, 74(3), 97-109.
Single runs of RP-HPLC procedure were used for simultaneous and organ-wise quantification of the terpenoid indole alkaloids (TIAs) serpentine, ajmalicine, vindoline, catharanthine, vincristine and vinblastine in whole adult plants of Catharcmthus roseus, sampled from 81 accessions and F2 generation of a cross. The average total TIA content was 159 mg distributed among stem, root, leaf and inflorescence organs in 13:8:3:1 proportion. The root and stem barks, leaf-petiole and corolla petals were several fold TIA-richer than root and stem woods, leaf-lamina and rest of flower organs, respectively. TIAs were undetectable in seed but present in the seedling. Quantitatively, serpentine (+ajmalicine), serpentine and catharanthine, vindoline, ser
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