000997 Bhaskar N;Sakhare P Z;Suresh P V;Gowda L R; Mahendrakar N S (Central Food Technological Research Institute, , Mysore-570 020, Email: firstname.lastname@example.org) : Biostabilization and preparation of protein hydrolysates from delimed leather fleshings. J scient ind Res 2007, 66(12), 1054-63.
Wet leather fleshings (LFs) from sheep and goatskins had high alkaline pH (12.1) and ash (18.1%) due to liming of fresh skins for leather production. Treatment of limed fleshings with 0.1% H2O2 (1:10 v/v) followed by 0.2 MCI (1:10 w/v) solutions produced very little or no H2S. Delimed fleshings, mixed with 19.5% (v/w) Pediococcus (iciclolactici culture, 20% (w/w) sugar and 2% (w/w) common salt, on fermentation at 30±2°C yield silage (protein 7%, fat 8% and ash 3.6%), which can be used in animal feeds. Optimized conditions (enzyme, 1.5% v/w of Protex-6L: temp., 55°C: hydrolysis time, 180 min) for liydrolyzing delimed LFs were determined by RSM. LFs yield white hydrolysate (yield, 3.5%; nitrogen, 12.4%; and fat, 1.5% w/w), which can be used in aquaeulture and animal feeds to supplement arginine, phenylalamine and tyrosine.
5 illus, 10 tables, 35 ref