000484 Sahoo S;Mishra S R;Nayak A;Ellaiah P (University Dep of Pharmaceutical Sciences, Utkal Univ, Vani Vihar) : Bioprocess intensification by nutritional optimisation for production of hyaluronidase by Streptococcus mitis MTCC *2695 species. J microbial Wld 2008, 10(1), 29-37.
Studies on process and nutritional parameters for the production of extracellular enzyme Hyalu-ronidase employing Streptococcus mitis MTCC *2695, was carried out under submerged fermentation and assayed for enzymatic activity. The effects of initial pH, time course profiles of pH, cell mass and hyalu-ronidase activity were studied. Different nutritional parameters including growth factors such as effect of vitamins, amino acids, growth hormones, various nutrient medium as source of complex nitrogen, trace elements, surfactants, chelators, solvents and antibiotics were investigated for enzymatic activity. The maximum enzymatic activity was obtained with initial pH at 5.8, incubation time for 48 h. Results of nutritional parameters showed that thiamin, 1-arginine, kinetin, sodium molybdate and N, N, N', N'-Tetraacetyl ethylene diamine showed best activity among vitamins, amino acids, growth hormones, trace elements and chelators respectively in the production medium containing hyaluronic acid (substrate), casein enzymatic hydrolysate as organic nitrogen source, magnesium sulphate salts, and carbohydrate sugars with initial and final pH (5.8 and 6.2) respectively. Sodium dodecyl sulphate, cadmium chloride, dimethyl formamide (20%) and clarithromycin showed strong inhibitory effect on hyaluronidase production among surfactant, trace elements, solvents and antibiotics respectively.
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