025006 BANO H, BHAT J I A, SIDDIQUI M A A, NOOR F, BHAT M A, MIR S A (Environmental Sciences Div, Sher-e-Kashmir Univ of Agricultural Sciences and Technology, Shalimar - 190 025, Email: firstname.lastname@example.org) : Seed germination studies of Saussurea costus Clarke, a step towards conservation of a critically endangered medicinal plant species of north western Himalaya. Plant Arch 2018, 18(1), 963-8.
Saussurea costus is critically an endangered medicinal plant species of North Western Himalaya. Its export has been prohibited as it comes under the category of red list species, according to Appendix 1 of CITES (CITES 2003). The species has been used in traditional healthcare systems of the region since times immemorial. The most widespread uses of the species include treatment of cough, cold, stomachache, toothache, ulcer and rheumatism. The roots of the species are used as an antiseptic and in controlling bronchial asthma. The Costus Oil is used in high-grade perfumes and as hair oil. It is also effective in the treatment of leprosy. In this study an attempt was made to examine the role of various treatments to overcome seed dormancy and enhance germination percentage for its effective conservation of Saussurea costs Clarke, a critically endangered medicinal plant species of Kashmir Himalaya. Seeds were given different physical and chemical treatments and germination was observed up to 44th day. Germination started and completed by different dates in different treatments. In treatments ch1 , ch2 , ch3 (30, 40 and 50 day chilling treatments), K1 , K2 (Treatments treated with different concentrations of potassium nitrate, K1 = 0.2 %, K2 = 0.3 %) and Control germination started and ended on different days but all these treatments took 30 days for complete germination. The best results were obtained in 50 days chilled seeds with 82 per cent germination followed by 80 % and 72 % germination in treatments GA31 (Gibberllic acid = 10-3 M) and GA32 (Gibberllic acid = 10-4 M), respectively. The chilling treatment ch3 and treatment GA31 were at par with each other but were statistically different fromall other treatments. Treatments GA31 and GA32 with 80 % and 72 % germination, respectively were statistically different from each other. Treatments K1 and K2 were at par with each other. 24 per cent germination was observed in control treatment, but no germination was observed in seeds treated with concentrated H2SO4. All treatment means except treatments S1 revealed significant differences when compared with control. Results also revealed that the rate of germination of seeds which were given different chemical treatments was enhanced in comparison to the control treatment. The coefficient of variation and mean ± standard deviation across days varied from 48.41 to 230.86 and 1.18 ± 2.72 to 51.36 ± 24.84, respectively.
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